1. Educational Background

2011/09-2017/05, PhD in Veterinary Medicine, key laboratory of animal physiology and biochemistry, Nanjing Agricultural University, P.R. China

2000/09-2003/06, M.S. in Biochemistry and Molecular Biology, Shanxi Agricultural University, P.R. China

1996/09-2000/06, B.S. in Veterinary Medicine Shanxi Agricultural University, P.R. China

2. Professional Experience

2022/05- Present, Vice director of Animal Mycoplasma Research Center, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Researcher

2017/08 –2022/05, Director of Lab. of Ruminant disease prevention and control, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Researcher

2015/08 –2017/08, Vice director of Lab. of Porcine disease prevention and control, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Researcher

2013/02 –2015/08, Vice director of Lab. of Porcine disease prevention and control, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Associate Researcher

2010/08- 2013/02, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Associate Researcher

2006/08 – 2010/08, Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences, Assistant Researcher

2003/10 – 2006/08, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Research Assistant

3. Professional Memberships

Member of the International Organization for Mycoplasmology (IOM)

Member of the Chinese Society of Microbiology

Member of the Chinese Society of Animal Husbandry and Veterinary Medicine

4. Expertise/Research Area

Research on vaccine, detection and prevention technology, pathogenesis of sheep & goat and porcine respiratory disease, esp. Mycoplasma hyopneumoniae, Mycoplasma ovipneumoniae, Mycoplasma bovis, Caprine Para-influenza virus type3, etc.

5. Publications

[1] Sun Min*;Li Wenliang;Zhang Wenwen;Yang Leilei;Hao Fei;Li Jizong;Mao Li;Jiang Jieyuan; Liu Maojun*. Screening interferon antagonists from accessory proteins encoded by P gene for immune escape of Caprine parainfluenza virus 3. Veterinary Microbiology, 2021,254: 108980

[2] Li Jizong, Yang Leilei, Mao Li, Li Wenliang, Sun Min, Liu Chuanmin, Xue Tao, Zhang Wenwen, Liu Maojun*, Li Bin*. Caprine parainfluenza virus type 3 N protein promotes viral replication via inducing apoptosis .Veterinary Microbiology, 2021,259:109129

[3] Yu Y, Wang H, Wang J, Feng Z, Wu M,Liu B, Xin J, Xiong Q*, Liu M*, Shao G. Elongation Factor Thermo Unstable (EF-Tu) Moonlights as an Adhesin on the Surface of Mycoplasma hyopneumoniae by Binding to Fibron. Front. Microbiol. 2018,9:974.

[4] Maojun Liu, Gaimei Du, Beibei Liu, Yun Hu, Jie Liu, Yimin Jia, F. Chris Minione, Guoqing Shao, Ruqian Zhao*. Cholesterol exacerbates Mycoplasma hyopneumoniae -induced apoptosis via stimulating proliferation and adhesion to porcine alveolar macrophages. Veterinary Microbiology,2017,211:112-118

[5] Liu M, DU G, Zhang Y, Wu Y, Wang H, Li B, Bai Y, Feng Z, Xiong Q, Bai F, Browning GF, Shao G. Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65. Vet Med Sci. 2016 , 78 (8) :1319-1322

[6] Gai M. Du, Jie G. Wu, Bi P. Luo, Zhi H. Hu, Liu A. Li, Mao J. Liu*. RNAi-mediated Ghrelin affects gastric H⁺–K⁺-ATPase activity and expression of GOAT–Ghrelin system in vitro. General and Comparative Endocrinology. 2016. 228:48–52.

[7] M.J. Liu, G.M. Du, F.F. Bai, Y.Z. Wu, Q.Y. Xiong, Z.X. Feng B. Li G.Q. Shao. A rapid and sensitive loop-mediated isothermal amplification procedure (LAMP) for Mycoplasma hyopneumoniae detection based on the p36 gene. Genetics and Molecular Research. 2015,14 (2): 4677-4686

[8] Zhiyuan Sun^#, Maojun Liu^#, Huafeng Zou, Xian Li, Guoqing Shao, Ruqian Zhao^*. Vaccination inhibits TLR2 transcription via suppression of GR nuclear translocation and binding to TLR2 promoter in porcine lung infected with Mycoplasma hyopneumoniae. Vet Microbiol. 2013,27, 167(3-4): 425-433. (co-first author)

[9] G.M. Du*, M.J. Liu*, N. Parvizi d, R.Q. Zhao. Ectopic expression of ghrelin affects gastric H⁺–K⁺-ATPase activity and expression of GHR/IGF-1 system in weaned mice. Regulatory Peptides. 2013,186:12-17.(co-first author)

[10] GAI-MEI DU*, MAO-JUN LIU*, YU-ZI WU, QI-YAN XIONG, FANG-FANG BAI Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Mycoplasma hyorhinis. Clin. Lab. 2013; 59(11-12): 1363-1371. (co-first author)

[11] Maojun Liu, Yue Zhang, Gaimei Du, Yun Bai, Haiyan Wang, Ping Jiang, Guoqing Shao, Analysis of Mycoplasma hyopneumoniae P65 gene prokaryotic expression and immunogenicity,      Chinese Journal of Animal and Veterinary Sciences.2014, 45 (2): 262-267

[12] Maojun Liu, Yue Zhang, Yun Bai, Haiyan Wang, Guoqing Shao, Preparation of Monoclonal Antibody against P65 Protein of Mycoplasma hyopneumoniae. Agricultural Science & Technology. 2014,15(11):1872-1875,1907.

[13] Maojun LIU, Dongmei LI, Guodong SU, Yongqi ZHOU, Fangfang BAI, Yuzi WU, Beibei LIU, Guoqing SHAO. Development and Application of A Quantitative Competitive PCR Assay for Detecting Mycoplasma hyopneumoniae. Agricultural Science & Technology. 2015,16(5):940-944.

[14] Zhanwei WANG*, Mao jun Liu*, Zhixin FENG, Li zhong HUA,Jixian XIE, Guoqing SHAO*. A New Model of Recycling Agricultural Production. Agricultural Science & Technology. 2013,167(3-4):417-24

[15] Maojun Liu, Yongqin Zhu,Zhixin Feng, Xusu WU, Guoqing SHAO*. Secretory Expression of Mycoplasma hyopneumoniae P97 R1 Gene in Pichia pastoris and Primary Application of the Expression Product. Agricultural Science & Technology, 2013,14(5):710-715

[16] LIU Mao-jun, SU Guo-dong, ZHOU Yong-qi, FENG Zhi-xin, WANG Hai-yan, ZHU Xiao-wei, SHAO Guo-qing*. Safety Study of Mycoplasma hyopneumoniae, Live Vaccine(168 Strain, Chinese Journal of Veterinary Drug. 2012.46(7):1-4

[17] Liu Mao-jun¹, DU Hai-xia¹, DU Gai-mei, CHE Qiao-lin, ZHANG Yue, XIONG Qi-yan, BAI Fang-fang,WANG Hai-yan, FENG Zhi-xin, SHAO Guo-qing*Truncated expression of P159 of Mycoplasma hyopneumoniae and identification of its adhesion activity, Chinese J Pre Vet Med , 2012,4(7):1-4

[18] LIU Mao-jun, WANG Zhan-wei, WEI Yan-na, MA Qing-hong, YANG Li-li, ZHOU Yong-qi, SHAO Guo-qing*. Establishment and Application of Double PCR Method for Detection of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis ,Chinese Journal of Veterinary Drug,2012,47(9):7-10.

[19] LIU Mao-jun , DING Zhi-yo ng , LIU Dong-x ia1, DU Hai-x ia, MIAO Fen-fang , GAN Yuan1,KONG Meng , FENG Zhi-xin , XIONG Qi-yan , BAI Fang-fang , DU Gai-mei , SHAO Guo-qing*. Mycoplasma hyopneumoniae DnaK Gene and ELISA Method, Journal of jingling institute of technology ,2011,27(4):79-84

[20] LIU Mao-jun, SHAO Guo-Qing, ZHANG-Yi. Sequence Analysis of Adhesin Gene of Different Mycoplasma hyopneumoniae Strains, Jiangsu Journal of Agricultural Sciences, 2010,26(3)：522-525

[21] Maojun Liu, Lianye Miao,Yongqian Zhao, Guodong Su, Yongqi Zhou, Jichun Wang, Peiyuan Sun, Guoqing Shao*, Live Mycoplasma hyopneumoniae vaccine(168 strains) clinical trial,. Animal husbandry and veterinary medicine,2009.41(8):93-95

[22] LIU Mao-jun, ZHANG Ying, SHAO Guo-qing, et al. Establishment of PCR Method for Detecting the Suspicious Sample of Mycoplasma hyopneumoniae, Journal of shanxi agricultural university (natural science edition), 2009.29(5):444-447

[23] LIU Mao-jun, GAN Yuan, TANG Hong-hui, SHAO Guo-qing, FENG Zhi -xin, WANG Hai -yan, SUN Pei -yuan, Detection of Classical Swine Fever Virus and Porcine Reproductive and Respiratory Syndrome Virus in Cases of High Fever Swine in Jiangsu, Journal of jinling institute of technology, 2009.25(4):84-88

[24] LIU Mao-jun, SHAO Guo-qing, ZHOU Yong-qi, SU Guo-dong, WANG Ji-chun, SUN Pei-yuan, FENG Zhi-xin, LIU Dong-xia ,Isolation and Identification of a Virulent Strain of Mycoplasma hyopneumoniae, Jiangsu Journal of Agricultural Sciences ,2009.25(2):308-310

[25] Gai-Mei Du, Mao-Jun Liu*, Zhong-Ming Chen, Ghrelin stimulates gastric acid secretion by rat gastric mucosal cells in vitro,World Chinese Journal of Digestology, 2009,34:3486-3490.

[26] Maojun Liu, Guoqing Shao *, The diagnosis of swine enzootic pneumonia Veterinary Orientation, 2009,(12):18-20

[27] LIU Mao-jun, LI Dong-mei, SHAO Guo-qing, FENG Zhi- xin,WU Xu-su, GAN Yuan, LIU Dong-xia, Establishment of PCR Method for the Identification of Mycoplasma hyopneumoniae, Journal of jinling institute of technology, 2008.24(3):99-101

[28] LIU Mao-jun, JIN M in, DU Gai-mei, SHAO Guo-qing, ZHANG Ying, Establishment of Indirect ELISA for Detection of Mycoplasma hyopneumoniae, Jiangsu Journal of Agricultural Sciences, 2007.23(5):437-441

[29] Maojun Liu, YongQi Zhou, Meijuan Ding, Guodong Su, Guoqing Shao, Vice Haemophilus pig blood antibody indirect hemagglutination assay, Swine industry science, 2007,(12):23-25.

[30] LIU Mao-jun, SHAO Guo-qing, ZHANG Ying, NIE Xiang-ting, Cloning and Expression of R1 Region of P97 Gene in Mycoplasma hyopneumoniae, Jiangsu Journal of Agricultural Sciences, 2005, 21(3):207-211.

6. Patents

[1] Expression of Mycoplasma hyopneumoniae P97 R1 in Pichia pastoris; People's Republic of China State Intellectual Property Office. ZL200910027159.1. Rank:1.

[2] Preparation and application method of LAMP kit for detecting Mycoplasma hyopneumoniae. People's Republic of China State Intellectual Property Office. ZL201110033271.3. Rank:1.

[3] A recombinant antigens ELISA test kit to detect Mycoplasma hyopneumoniae antibody. People's Republic of China State Intellectual Property Office. ZL201210244908.8. Rank:1.

[4] The pig mycoplasma pneumoniae vaccine strains; People's Republic of China State Intellectual Property Office. ZL201310608564.9. Rank:1.

[5] The aplication of pig mycoplasma pneumoniae vaccine strains; People's Republic of China State Intellectual Property Office. ZL201310605986.0. Rank:1.

[6] A haemophilus parasuis vaccine strains serum type 5 . People's Republic of China State Intellectual Property Office. ZL201310125433.5. Rank:1.

[7] A virulent strain of Mycoplasma ovipneumoniae and its application. People's Republic of China State Intellectual Property Office. ZL202110341911.0. Rank:1.

[8] A strain of Mycoplasma ovipneumoniae and its application in the screening of anti-Mycoplasma ovipneumoniae preparations. People's Republic of China State Intellectual Property Office. ZL202110341911.0. Rank:1.

[9] Attenuated live vaccine against mycoplasmal pneumonia of swine (MPS) and use thereof. The united States of America Intellectual Property Office. US 9849168 B2. Rank:3.

[10] Swine mycoplasmal pneumonia of attenuated vaccine and use thereof. European patent office. EP 3098301 B1. Rank:3.

[11] Duplex fluorescence quantitative RT-PCR kit for differential detection of NSs-deletion type from wild-type of rift valley fever virus. Republic of south Africa Patent Office. 2020/04451 PA174636/ZA. Rank:9.

7. Research Grants

[1] National Key Research and Development Program of China (2022YFD1800603): Research on new biological product quality control technology and reference materials, Affiliated Project: Development of reference materials for veterinary biological products; 2022.11-2025.12.

[2] The 333 High-level Personnel Training Project of Jiangsu Province of China (BRA2019092), Research on rapid detection technology of Mycoplasma ovipneumoniae and Caprine Parainfluenza Virus Type 3 2019.7-2021.6.

[3] Sub-Program of National Key Research and Development Program of China (2018YFD0502106-4), Integration and demonstration of prevention, control technology and safe drug use technology for major sheep epidemics, Affiliated Project: Application and demonstration of efficient and safe breeding technology of sheep; 2018.6-2020.12

[4] Sub-Program of National Key Research and Development Program of China (2018YFD0502006-4),Study and demonstration of immunization program and integrated disease prevention and control technology for regional goat farm, Affiliated Project: Application and demonstration of efficient and safe breeding technology of goats; 2018.6-2020.12.

[5] Jiangsu Agricultural Science and Technology Fund (CX(18)3019), Research and development of Caprine Parainfluenza Virus Type 3 vaccine. 2018.7-2021.6.

[6] National key laboratory of veterinary biotechnology open issue. Screening the virulence factors of Mycoplasma hyopneumoniae. 2015-2017.

[7] Jiangsu Agricultural Science and Technology Fund(CX(13)3066),The development of mycoplasmal pneumonia inactivated vaccine with aqueous adjuvant. 2013.7-2016.6

[8] National Natural Science Foundation of China (31100136), Comparative proteomics Analysis of Porcine Tracheal Ciliated Cells infected by Pathogenic Strain and A virulent Strain of Mycoplasma hyopneumoniae. 2012.1-2013.12,

[9] Jiangsu Agricultural Science and Technology Fund (CX(11)3025), The standardization pattern in establishing the middle and small intensive pig farms. 2011.7-2012.6

[10] Jiangsu Agricultural Science and Technology Fund (CX(12)5051 ), Comparative proteomics Analysis of the Porcine Tracheal Ciliated Cells infected by Pathogenic Strain and A virulent Strain of Mycoplasma hyopneumoniae. 2012.7-2014.6

[11] Jiangsu Agricultural Science and Technology Fund (cx(09)118), The Screening research on the genetic engineering vaccine candidate Protein of Mycoplasmal pneumonia. 2009.7-2011.6

[12] The provincial standard of Jiangsu Province (5510925), the diagnostic techniques of Mycoplasma hyopneumoniae of swine. 2010.7-2011.6

[13] The provincial standard of Jiangsu Province (5510926), the diagnostic techniques of Glasser‘s disease. 2010.7-2011.6

[14] The provincial standard of Jiangsu Province (5510830), The PCR detection method of Mycoplasma hyopneumoniae. 2008.7-2009.6

[15] The open project of Jiangsu Provincial Key Laboratory of animal products safety research (4910612C), Rapid detection method for Toxoplasma gondii in pork zoonosis pathogen. 2008.7-2009.6

[16] Research foundation of Jiangsu Academy of agricultural science (0610808-3), vaccine of Swine mycoplasma pneumonia. 2008.7-2009.6

[17] Research foundation of Jiangsu Academy of agricultural science (6110640), Research on immunization with inactivated vaccine of Mycoplasma hyopneumoniae. 2006.7-2008.6

8. Honors & Awards

[1] 2023,Third prize of Scientific and technological progress in Hebei Province. Rank 7.

[2] 2022, The “333” talents project in Jiangsu Province

[3] 2022, Second prize of Science and Technology Award of Jiangsu Animal Husbandry and Veterinary Society, Rank 1

[4] 2022, First prize of the Science and Technology Award of Jiangsu Agricultural Society, Rank 2

[5] 2022, Second prize of Science and Technology Award of Jiangsu Animal Husbandry and Veterinary Society, Rank 8

[6] 2021, Excellent Innovation Team Award of China, Agricultural Science and Technology Award, Rank 3.

[7] 2021, Young and Middle-aged Experts with Outstanding Contributions in Nanjing.

[8] 2020, First prize of the Science and Technology Award of Jiangsu Academy of Agricultural Sciences, Rank 8

[9] 2020, Second prize of Science and Technology Award of Jiangsu Academy of Agricultural Sciences, Rank 9

[10] 2016,The “333” talents project in Jiangsu Province

[11] 2016, The 15^th Jiangsu Youth Science and Technology Award and the "Top Ten Young Science and Technology Stars" in Jiangsu Province

[12] 2015, Second prize of National Award for Technological Invention, Rank 3.

[13] 2013, Annual award of science and technology of Jiangsu Academy of Agricultural Sciences, Rank 1

[14] 2011, First prize for China Agricultural Science and Technology Award, Rank 2.

[15] 2011, Golden Bridge Awards, Rank 2.

[16] 2011, Excellent Patent Awards of Jiangsu Province. Rank 2

[17] 2009, the Nanjing municipal outstanding patent award. Rank 3.

[18] 2008, Second prize of Scientific and technological progress in Jiangsu Province. Rank 3.

[19] 2007, Second class of national new veterinary drug registration certificate,Rank 3.